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KMID : 0360220240650020139
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Journal of the Korean Ophthalmological Society
2024 Volume.65 No. 2 p.139 ~ p.144
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Effect of Latanoprostene Bunod on the Permeability of Trabecular Meshwork Cells
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Park Hyeong-Seok
Kim Jae-Woo
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Abstract
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Purpose: To investigate the effects of latanoprostene bunod (LBN) on nitric oxide (NO) production and permeability in human trabecular meshwork cells (HTMC).
Methods: HTMC were treated with 50 and 100 μM LBN and latanoprost free acid (LAT) for 30 minutes. Additionally, 100 μM LBN was co-exposed to 0.5 mM L-NAME (N-Nitroarginine methyl ester). Cellular viability and NO production were measured using MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) and Griess assays. The permeability and resistance of the HTMC monolayer were evaluated by trans-endothelial electrical resistance (TEER) and carboxyfluorescein permeability.
Results: Exposure to 100 μM LBN led to increased NO production, whereas co-exposure to L-NAME reduced NO production.
Treatment with 100 μM LBN decreased the TEER of the HTMC monolayer. LBN exposure heightened carboxyfluorescein permeability, but co-exposure to 100 μM LBN and L-NAME reduced permeability. LAT treatment did not affect NO production or permeability.
Conclusions: LBN increased the permeability of the HTMC monolayer and increased NO production. Therefore, LBN might increase trabecular outflow in addition to promoting uveoscleral outflow.
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KEYWORD
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Latanoprost, Latanoprostene bunod, Nitric oxide, Permeability, Trabecular meshwork
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